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Optical imaging inherently offers many advantages which are well suited for evaluating the study of cell and tissue engineering: (1) higher spatial resolution and sensitivity, (2) tissue and cellular information based on scattering, absorption, and fluorescence, and (3) availability of contrast agents for specific targeted biomarkers. Among various optical imaging modalities, optical coherence tomography (OCT) is the most emerging imaging technology which allows real-time cross-sectional imaging with high resolutions. OCT could be a very promising technique which has the potential to be used as an adjunct to histological tissue observation when it is not practical to take specimens for histological processing, when large areas of tissue need investigating, or when rapid microscopic imaging is needed. This presentation will describe the use of OCT as an advanced imaging tool for fast tissue screening and directed histological tissue sectioning. The material covers the latest work of label-free optical imaging using optical coherence tomography (OCT) for large-field of view tissue imaging. Specifically, the talk will highlight quantitative OCT for evaluating artificial blood vessel and engineered skin. Long term objective of these researches is to develop high resolution and complementary imaging systems to better assess the specimens in tissue engineering research and to enable applying it to dynamic study.
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